Dear Mohammad,

You will find a good answer in the following links

https://socratic.org/questions/how-to-calculate-binding-energy-by-j-u

http://formulas.tutorvista.com/chemistry/binding-energy-formula.html

With my best regards

Prof Bachir ACHOUR

 Dear Mohammad Alqam, 

Yes , we can use the documents proposed by Professor Bachir Achour. I recommend it. But i add something: the energy bending between two apshaltene can also determined by other methods by heat gained after bend destruction, or the atomistic methods, etc.

Hope, helping to solve your question.

Best regards!

Link:

http://byjus.com/binding-energy-formula