30 September 2024 1 4K Report

What can be used to block INS which help in IAA synthesis

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Problem with beta galactosidase staining assay: X-gal crystals in staining solution?

Hello, I'm conducting a beta galactosidase staining assay for my fibroblasts using X-Gal. I dissolve X-Gal (stock 50mg/ml prepared in DMSO) in the staining buffer at a final concentration of...

25 March 2024 8,799 3 View

If OIBs are uncommon during Archean due to higher melting degrees, why Carbonatites are found during Archean?

The absence of Ocean Island Basalts (OIBs) in the Archean rock records is commonly attributed to the higher melting degrees associated with mantle plumes. However, this explanation raises...

14 March 2024 2,195 1 View

What is the best method for endophytic bacterial isolation?

Mostly bacteria living inside plant roots.

14 March 2024 4,602 0 View

Counterpoise (BSSE) Correction for more than 2 fragments?

What is the correct way to calculate the BSSE correction for a molecule having 3 fragments? Please see the attached image for details. I know the values are pretty close but still I would love to...

25 October 2023 3,990 1 View

Can I use TRIX index for coastal lagoon using DIN and PO4 instead of TN and TP?

Can I use DIN and PO4 in the given formula : The trophic index (TRIX) used in the present work has been given by the following formulation (Vollenweideret al.1998): TRIX = [log(Chla∙ (D%)O2 ∙ TN ∙...

26 September 2023 432 1 View

What was the mode of weathering/erosion during Hadean to Mesoarchean?

Many think that, because of the vastness of the oceans, the continents were underwater throughout the Hadean and much of the Archean periods. It wasn't until the Mesoarchean that continents began...

19 September 2023 6,875 0 View

Has anyone studied locus specific 5-hydroxymethylation using Epimark 5mC and 5hmC kit?

I am trying to quantify 5mC and 5hmC using Epimark 5mC and 5hmC kit from NEB. I did the end-point PCR after glucosylation and digestion with MspI and HpaII. It didnt generate a a yes or no answer,...

06 September 2023 6,221 0 View

How to calculate the number carbon quantum dots (CQDs) internalized per cell using "IntraCell V2" plugin in ImageJ?

How can the number of carbon quantum dots (CQDs) internalized per cell be calculated using the "IntraCell V2" plugin in ImageJ?

01 September 2023 419 0 View

Why the EPMA analysis for FeO for native metal (Iron) is coming as 110, 124, 114 (wt%) etc.?

The EPMA analysis for FeO for native metal (Iron) is coming as 110, 124, 114 (wt%). What could be the possible reason?

20 July 2023 2,583 0 View

What is the unit of polarization resistance (Rp) and Area Specific Resistance(ASR) for symmetric SOFC ?

I need to calculate oxygen exchange current density using polarization resistance.

07 June 2023 9,237 2 View

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Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

I have two problems: 1) the enzyme is not immobilizing efficiently into the MOF material.. 2) the MOF itself has peak on 400nm by using p-NPA test.?

I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...

20 July 2024 1,440 3 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View

How does the catalase enzyme contribute to the process of photosynthesis in plants?

كيف يساهم انزيم الكاتلاز في عملية التمثيل الضوئي في النباتات؟

09 July 2024 7,512 1 View

What is the role of catalase enzyme in plants?

ماهو دور انزيم الكاتليز في النباتات؟

09 July 2024 5,824 2 View

What is the protocol for enzyme freeze drying?

what is the protocol for enzyme freeze drying?

09 July 2024 6,464 6 View

How do I make 10 cc of enzyme solution with a concentration of 0.2 units/mL?

Hi We have alpha-amylase enzyme that written 5 units /mg protein on its container. How do I make 10 cc of enzyme solution with a concentration of 0.2 units/mL?

08 July 2024 2,285 2 View

Please describe clear and elaborate process of separating solids from garbage enzyme/ eco enzyme after 90 days of fermentation.?

How we should screen out the solid material left after 90 days of fermentation in making of garbage enzyme. Is there any standardized process.

17 June 2024 4,840 3 View

Diluting an enzyme before an experiment?

What is the reason or importance of diluting an enzyme before an experiment? I'm writing a report on enzyme activity and characterisation and i need help with understanding this: we were told to...

06 June 2024 8,714 2 View

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