I need to send a collaborator mounted cryosections of spinal cord tissue. The slides are stored at -80C here in the lab. What is the best way to ship? I've sent on dry ice, and that seems to work OK, but several times now, the recipients have told me that the sections easily fall off the slides with washes. That never happens in house.
What about shipping in 100% ethanol? If ISH (RNAscope) is the goal, that seems like it might be appropriate. What do you think?
Did you use coated slides?
The best way is to wrap each slide in Aluminium foil and then send it on dry ice.
You are doing the right way of shipping. If the person on the other end has experience of handling cryo sections:
Before immunostaining, leave the sections at 4 degree centrigrade overnight, the OCT will melt and sections will settles down to slide surface, next morning leave slides at RT for 10 minutes, washing has to be very gently with cryosections.