How to avoid the extraction of Arsenobetaine (AB)?

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Why do we observe different morphology of MCF 7 cells?

Hello, I have been using MCF 7 cell for 3 months. The newly thawed cell vial was observed to have cells with different morphology. These round shaped cells are also attached to the flask. However...

19 July 2023 6,057 2 View

What are the EU requrment of DOC (Declaration of Conformity) when the manufacture from outside EU?

Is it necessary to European representative detail in DOC when the manufacture from out side the EU region. The product here I mentioned in not in the category of MD or IVD. Just use for food and...

12 August 2022 7,956 1 View

Can road humps be used to discharge traffic with constant headways (or at regular intervals) in heavy traffic flow situations? Any studies known?

Are there any studies done regarding this phenomenon?

01 September 2021 9,383 3 View

Formaldehyde Levels in Fish?

(1) What are the regulations (already available) for Formaldehyde level in fish? (2) What is final standard published in FSSAI?

14 May 2021 9,334 3 View

What are the recent literature for a Buddhist theory of Public Relations?

I am on the study of modelling a theory of Buddhist Public Relation based on the cultural Public Relations. though there are some asian communication theories of Prof. Wimal Dissanayaka, Prof....

11 November 2016 4,803 3 View

What is the best polymerase and approach for amplifying 6kb gene from commercially obtain cDNA?

Hi all, So I want to clone out a gene from a commercially obtained cDNA library. I was wondering if people have been successful using a nested primer approach for a gene of almost 6Kb? I have...

26 July 2016 1,876 3 View

What is the proximate composition of Chitala ornata (knife fish) flesh?

Do you any reference/literature of the proximate composition, especially protein content of Chitala ornata (Knife fish) flesh?

02 May 2016 3,085 0 View

I have following question in T-bars analysis. Please give me your support to solve that?

T-bars analysis of fish and fishery products. I have following question in T-bars analysis.Please see the attached document

05 April 2016 538 0 View

Can you help me to the calculate following redox calculation of titartion vitamin C?

Here, I attached one method published for vitamin C determination, in generally Iodine solution used, but here, they use KI & KIO3? How to calculate I2 mol in here without standerdization or...

16 February 2016 1,123 3 View

Anybody have a chromatogram of Dr. Ehrenstorfer organochlorine pesticides mix 2 (18 pesticides) run in DB 608 column, GC-ECD?

Anybody have a chromatogram of Dr. Ehrenstorfer organochlorine pesticides mix 2 (18 pesticides) run in DB 608 column, GC-ECD? Because, I have a little doubt in my standard mix, hence I need to...

12 February 2016 3,770 0 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Basis set and input instruction to calculate HOMO and LUMO using Orca for Imidazolium based organic salts?

Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...

09 August 2024 5,444 7 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

How to improve peaks separation in HPLC?

Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...

30 July 2024 435 3 View