To establish a relationship between the force applied during orthodontic tooth movement and variation in the pulpal blood flow.
According remodelling process the variation depepond on the magnitude of force level within normal physiological value
Dear Ashok Ayer, the most used form is by measured by laser Doppler flowmetry already used by Sano et al. (The effect of continuous intrusive force on human pulpal blood flow); Mac Donald and Pitt Ford (Blood flow changes in permanent maxillary canines during retraction); Barwick and Ramsay (Effect of brief intrusive force on human pulpal blood flow); Brodin et al. (Instant assessment of pulpal blood flow after orthodontic force application); Babacan et al. (Pulpal blood flow changes due to rapid maxillary expansion); and Ikawa et al. (The effect of short-term tooth intrusion on human pulpal blood flow measured by laser Doppler flowmetry).
There is also a further method which is by histomorphometric evaluation performed for example by Santamaria et al. (Initial pulp changes during orthodontic movement: histomorphological evaluation).
I hope your problem has been solved.
Best regards
Mild orthodontic intrusive force application in closed apex teeth causes no significant histological changes in adolescents and adults. However, it seems that inflammatory-related histological pulpal changes are more severe in adults after one month of intrusion. This emphasizes the importance of applying mild orthodontic forces with adequate rest intervals in older individuals.