For an engineering project, I have a sensor to measure a certain gas concentration. Unfortunately this sensor returns unitless values between 0-1023, and there is no decent alternative to it. In order to calibrate and understand the meaning of these values, I'm looking to compare the measured values to the theoretical concentration. This calculation is something I am not confident in performing myself and so I would like to ask for some advice here.

The working conditions are as follows:

A 3 liter airtight box, opened from above, with 2 identical pipe holes on opposite sides. Both holes are of 14.02 mm diameter. The gas flows in through one, at 250 ml/minute. The other hole allows the air to flow through it, to relieve the pressure in the chamber. The chamber at first is filled with normal ambient air. The gas that flows in is 95% ambient air, and 5% isoflurane, which is the gas I am measuring.

With this information, how can I calculate an estimation of the isoflurane concentration in the chamber, preferably in PPM?

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