I want to analyze TCA cycle, but pyruvic acid, glyoxylic acid cannot be detected. My GC MS is 7890A-5957C, HP-5MS column, MTBSTFA as derivatization reagent, Anyone can give some advice?
This is clear, both acids are subject to decomposition when injected into the heated column. Convert acids to the methyl esters by reaction with diazomethane (the reaction proceeds rapidly at RT)
Looking for reference mass spectra ? A whole searchable database of mass spectra of pure species of atmospheric interest (molecule and dervivatives) is available for download at the EUROCHAMP datacenter. Check out : https://data.eurochamp.org/data-access/mass-spectra/