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Hello, everyone. I found the bacteria I cultured can be inhabitated by another bacteria when I culture then together in the plate. Now, I want to analyze which metabolite contribute to the...
24 December 2018 6,967 2 View
I want to analyze TCA cycle, but pyruvic acid, glyoxylic acid cannot be detected. My GC MS is 7890A-5957C, HP-5MS column, MTBSTFA as derivatization reagent, Anyone can give some advice?
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When I use freeze-dryer, after freeze at -80C for 5 or 7h, drying it but there are always sample loss after drying, some of the dried sample get out of the glass tube. How to avoid the loss?
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After derivatized with MTBSTFA, some amino acids have 2 derivatives, like tryptphan, cysteine and threonine, defferent number of hydrogen were derivatived. Does anyone know the reason and how to...
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I am a beginner in R language and I want to analyze metabolomics data with R. Recently I found an R package "muma" which is for metabolomics data analysis but I don't know how to use that package....
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When I analyze 20 amino acids with GCMS, I use MSTFA to react with sample, but there are some combined peaks cannot be separate. Like proline-Isoleucine, and i cannot detect tryptophan and...
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