Do blood cells die after staining in blood smear analysis ?

This might be a very naive question but I've not been successful in finding sources that explicitly say that the cells are alive post staining. Do Romanowsky stains such as Giemsa, Wright etc....

09 September 2020 2,920 3 View

What is the lower limit for reliably counting cells in a typical hemocytometer ?

I am looking to quantify the counting errors associated with serial dilutions. Few sources mention a lower cap of 25,000 cells per ml - does this sound right ?

27 December 2019 3,714 4 View

I have made a flexible i.e. less stiffness NFRPMC, How to achieve stiff composite?

Hello All, I have made a laminate which is very flexible which can be cut into parts by bare hands. I cured it at normal room temperature for day and half by placing load on laminate during...

08 June 2018 9,110 3 View

Why is smear on gel in my pcr of C-DNA samples?

I am getting smear in the gel of my pcr of CDNA samples, and to surprice me, I am getting band in GAPDH +ve control but not in my CDNA samples... Also I incorporated 2 plasmids as +ve controls,...

17 December 2017 6,096 2 View

What should be the ignition temperature for determination of soil organic matter by LOI method using Muffle furnace?

In literature, different authors have used different temperatures and different incubation periods. So, kindly suggest the most appropriate temperature and incubation period for determination of...

24 June 2016 3,446 7 View

Can anyone give me the code to solve time dependent PDE's using Chebyshev Differential matrices ?

A simple one would suffice.

27 January 2015 3,642 2 View

Can anyone help me about multiple bands in my Western blot?

I did Western blot of samples from cotton root tissues from same event, but of different time spans (took samples after 1 month interval for 4 months), and I got difference in banding...

17 July 2014 4,181 6 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

How to interpret this FTIR spectrum of pyrolyzed hydrochar?

20 February 2021 2,840 3 View

What is error #2070 in gaussian.how we do resolve it?

We want to ONIOM calculation in gaussian16. However, gaussian doesn't work. What should we do? Atom 15 layer R linked to atom 85 is also bonded to atom 161 in layer M bad...

15 February 2021 7,766 3 View

Best protocol for immunogold using araldite resin ?

Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...

14 February 2021 4,959 1 View

How to improve the adhesion of epoxy coating on steel substrate? Which factor affects?

I am facing a problem regarding a de-adhesion problem with the coating. A brief description of my methodology is given below. I am using mild or Low Carbon steel as a substrate and I polish the...

14 February 2021 2,167 4 View