Hi Matin:

On one hand, you could do a complete metagenomic analysis through NGS massive analytical methods, from which you can get an approximation to the relative abundance of each taxa (OTU) in the consortium. Alternatively you could do quantitative PCR, but amplification should be done separately for bacteria and algae as the specific primers are different.

Dear Antonio,

Dear Jiu-Qiang,

Thank you very much for your useful information.

You are welcome Matin

Antonio

Hi Matin

you might get some ideas how to do this from Kleinteich et al.

1. Pole-to-pole connections: Similarities between Arctic and Antarctic microbiomes and their vulnerability to environmental change. Frontiers in Ecology and Evolution, 10.3389/fevo.2017.00137

Best of luck

Dan

Hi Daniel,

Thanks for your useful information.

Best,

Matin

Hi Matin,

I guess a simple method can be chlorophyll extraction with methanol and quantify that with a spectrophotometer, assuming that the chlorophyll content remains constant in your microalgae. If you have fluorescent plate reader you may not even require extraction. However, this method is not very accurate due to the assumption I mentioned.

Best,

Mohsen