I have done 3D DNA FISH of two gene probed with alexa fluor 488 and alexa fluor 594 respectively and counter staining with DAPI with good signal to background ratio and I want to calculate the distance of alleles from center or periphery . I have tried image j but it requires plugins like TANGO and I am unable to run plugins as it requires mongodb database to connect. I know software like IMARIS and huygens professional will do this job very easily but my university doesnt have that. so please suggest my how to calculate distance of loci from centre of the nucleus. I am attaching avi file for single color for reference.
Thank you
I have used TANGO in the past and had no problem installing it through the normal FIJI update route (Help > Update > Manage Update Sites > selecting TANGO). You don't detail the specific problems you ran into but if you continue to have trouble I would recommend you post on https://forum.image.sc which is a forum specifically for image analysis questions where a lot of very helpful people contribute regularly including some of the developers of TANGO and other plugins that may be useful to you.
Hello Laura,
I have installed MongoDB and configuration also is done but I am facing problem when it says connect to MongoDB data base. I tried to connect it but couldn't able connect it. From below points I am unable to move further.
PLEASE NOTE THAT THE CURRENT VERSION OF TANGO CURRENTLY ONLY SUPPORTS MONGODB VERSION 3.0.0, IF YOU HAVE A LATER VERSION, DOWNGRADE TO 3.0.0
TANGO needs a running MongoDB server, usually on the same computer. To install MongoDB as a service on your computer run the command Create MongoDB service in the menu TANGO/Configure of ImageJ. MongoDB will be automatically started at startup of the computer. When running this command, the default directory is written in the log page. Make sure there is enough available disk space to store the images you wish to analyse. On linux system you need to install xterm Alternatively, the server can be run on a distant server.
When mongoDB is install also Run the comamnd "Configure Database module"
Thank you
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