What is the most efficient way to adjust pH of the extracts for the Hedley Fractionation Method before colorimetric analysis? I am using 2M HCL and 5M NaoH, but still unable to adjust the pH and facing big problems in the analytical data. What should be the optimum pH range?
simply with NaOH (or lacking KOH) as the base and HCl as the acid
the normality of your base and / or acid depends on the initial pH of your soil and the pH you want to reach
if the difference in pH is greater than two units working with normalities of 1 N if the difference is less than two a pH of 0. N is advised
in any case, note the volume added
The optimum pH depends on the method you are using for color development before cliometric analysis. In general, we are using 0.1N NaOH and 0.1 N HCl to adjust pH, which works fine.
Dear Waqas
Please employ low-stength acidic/ basic ( HCl/ NaOH) solutions for narrow range pH adjustments. For broader pH adjustments (for ex. pH 5 to pH 9), high-stength solutions can be employed. otherwise you may face difficulty in pH adjustments.
simply with NaOH (or lacking KOH) as the base and HCl as the acid
the normality of your base and / or acid depends on the initial pH of your soil and the pH you want to reach
if the difference in pH is greater than two units working with normalities of 1 N if the difference is less than two a pH of 0. N is advised
in any case, note the volume added
Usage of HCl and NaOH solutions is the most popular method. Also you can use buffer solutions.
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