I purchased 10mm x100mm TSA w/5% sheep blood plates, with goal of culturing S.pneumoniae from mouse specimens. I would like to add gentamicin to these plates- does anyone have a protocol.
I am beginning to use OG+ in my district and noticed during training there are a lot of materials and routines, which may be overwhelming for my students, especially those on the autism spectrum....
30 July 2024 5,695 0 View
These will be used for the study of microplastics in the lung
29 July 2024 9,609 2 View
I am starting to work on this question, with particular reference to what we might be able to learn from countries outside the UK. Of course this topic is huge, and I am breaking sustainability...
25 July 2024 1,545 3 View
I am preparing to run a lot of samples on a 384 well plate and I wanted to test if any well in my PCR machine is dead so as not to lose any data. I would really appreciate help with how I can do...
24 July 2024 8,172 1 View
Looking for research articles regarding effective scholarship in EBP in nursing?
11 June 2024 1,896 4 View
Ethiopia has seen escalated civil war since 2021. Immediately after Ethiopia's PM took over power in 2018, a ceasefire and a peace agreement was signed between Ethiopia and Eritrea ending one of...
06 June 2024 6,306 0 View
I am looking forward to using random walks as a probability distribution for solving a particular problem in a research field. I found some blogs and articles which states different concepts of...
26 May 2024 8,302 0 View
I am looking for the full text of: Pfaffenrath V, Kaube H. Diagnostics of cervicogenic headache. Funct Neurol. 1990 Apr-Jun;5(2):159-64. PMID: 2227537. Hopefully someone can help me...
22 May 2024 9,624 0 View
Hi all, I used a ZymoPURE II Plasmid Maxiprep kit to isolate plasmid DNA and ended up with very low yield and poor quality. Specifically, less than 10ng/uL in 500uL from 150mL of culture, and...
15 May 2024 6,870 2 View
Hello, I am a PhD student and I need a help/ resources on how to clean and analyse OSPAN working memory task data! Thank you
08 May 2024 6,649 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View