Hi,
Are there any method that can accelerate the collagen hydrogel crosslinking process to make it simultaneously like alginate+Calcium cation reaction?
Thank you.
Hi, I am having problem of dissolving collagen (porcine) from powder. I would like to get the final concentration of 10 mg/ml or higher. Does anyone has a protocol for this? Currently, I use 0.1...
10 April 2019 4,497 7 View
In order to check cell activity on hydrogel scaffold, which in vitro assay is recommend? Will the absorption property of hydrogel affect the mention assay? Thanks.
11 December 2016 7,358 8 View
Hi, I recently have some problem with the HUVEC cells. They proliferate very slow. Can I add a bit of VEGF inside the medium to enhance the rate? Will it help? Thanks.
02 November 2015 5,435 16 View
I will need to remove dioxane form the solution. However, if I don't use rotary evaporator, Can i use open air-drying instead? Are there any other methods to eliminate dioxane form mixture or...
23 August 2015 8,629 3 View
Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
24 July 2024 3,937 0 View
Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...
19 July 2024 3,906 0 View
I need an appropriate positive control for WB antibodies I am using to confirm the presence of exosomes in samples. The thing is I am having reproducibility issues, and I would like distinguish...
19 June 2024 4,210 3 View
Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...
13 June 2024 9,473 0 View
im working on the photocrosslinking of hydrogels and want to check whether crosslinking happened with the material I have.
12 June 2024 5,579 3 View
I am intrested in the preparation of collagen carriers, dehydration, paraffin infiltration, and paraffin embedding. Thanks in advance.
05 June 2024 3,831 0 View
I started a cell culture with a mix of suspended and adherent cells (presumably fibroblasts) on May 10th. After a week, I treated them for adherent cells only. On May 24th, most of the adherent...
02 June 2024 4,903 5 View
i am working on self heating membrane preparation. so for crosslinking of membrane, may i use copper chloride, or magnesium chloride or any bi valent cation salt to crosslink it? and which one...
29 May 2024 2,474 0 View
I have been making hydrogels regularly using 10X DMEM (phenol red indicator), Ultrapure water, 1N NaOH, and 3.41 mg/ml Collagen (suspended with acetic acid). We have used the same calculations for...
29 May 2024 4,655 0 View
Type 1 diabetes, the effect of peer support in self care efficacy, emotional well being
27 May 2024 4,325 0 View