How to dissolve collagen powder to get the concentration of 10 mg/ml or higher?

Hi, I am having problem of dissolving collagen (porcine) from powder. I would like to get the final concentration of 10 mg/ml or higher. Does anyone has a protocol for this? Currently, I use 0.1...

10 April 2019 4,425 7 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

How do you prepare a porous crosslinked Polyvinyl alcohol and Glutaraldehyde membrane or film?

I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption

08 February 2021 7,493 3 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

How can I set up UV irradiation for crosslinking lab?

Hi, I am using a mix of 2 polymers and want to try and crosslink them. I have a UVA lamp of 365nm and have tried the lab previously at 30cm above. How would you recommend I approach the lab and...

31 January 2021 8,711 3 View

Why sometimes current- voltage plot in chemiresistors/ FET does not pass through the origin?

I am trying to measure resistance from I-V curve from a chemiresistor/ FET sensor with a keithly sourcemeter. The substrate is soaked/ wet with protein and phosphate buffer only. But the I-V line...

24 January 2021 8,009 3 View

How to measure CPT1/FAO activity in a rare cell type?

Hello all, I am searching for a way to measure the activity of CPT1 and/or FAO in mouse hematopoietic stem cells, which means I have very little material available to do so. Any...

20 January 2021 8,499 2 View

How can I verify the design on the FPGA ZedBoard?

I have designed AXI Interconnect to connect 4 masters and 1 slave using SystemVerilog and Vivado Tool. The simulation stage of the design is working well. In the design, there are more I/O pins...

06 January 2021 8,487 2 View

Transfecting high molecular weight poly I:C using lipofectamine 2000?

I'm planning to transfect HepG2 and Huh7 with poly I:C (high molecular weight poly IC from Invivogen using lipofectamine 2000 as transfectant reagent. I would like to do a standard curve using...

01 January 2021 239 1 View

How to remove the bubbles in Collagen gel and maintain the transparency?

I am working on the capillary formation of Lymphatic endothelial cells in a collagen matrix. But I am facing a technical problem to handle Collagen. I have made many times; I am not able to remove...

27 December 2020 5,495 3 View