Hi Akila,

a strong promoter in the T-DNA insert (for example 35S promoter for the selection marker) could affect the expression of flanking plant DNA, similar to activation T-DNA tagging. For this, the insert should be located in the promoter region.

Thank you Dr. Eric.

I am looking for illustrated T-DNA cassette of SALK, GABI-KAT and SAIL T-DNA. Have you come across any publication. (in addition to Dr. Ulker's paper; doi: 10.1111/j.1365-313X.2008.03608.x )

And comparing their advantages and disadvantages?

A disruption/ re-arrangement by T-DNA insertion in negatively regulating cis-acting element region may also ehance the transcriptional level of mRNA.

J Exp Bot. 2012 June; 63(10): 10.1093/jxb/err235

J Exp Bot. 2011 Nov; 62(15): 5531–5545