I need the oligomycin to be dissolved in Ethanol. However, I will need the final concentration of the ethanol in my samples to be 0.1%. I'm not sure whether oligomycin would still be soluble in this solution with 0.1% ethanol.
you have to dissolve oligomycin 0.1 gm in 100 ml ethanol. Depending on your need you will make a solution.
Oligomycin is dissolved in Ethanol.
https://www.caymanchem.com/pdfs/11342.pdf
Thank you. I do understand that. But it doesn't really answer my question. If I dilute the srock oligomycin down to what I need, in order not to effect my cells, the ethanol is 0.1%
Is oligomycin going to precipitate out? Or is it stable?
Hi Mihray,
I think it should remain in solution. I used to use around 0.001% ethanol in media and the oligomycin (0.05ug/ml) stayed in solution. I had diluted it from the same stock Parth described, so it should hopefully work for you too.
Say hi to the others for me!
All the best,
Paul
Thank you Paul. You've just saved me from a lot of trouble.
Will do,
Hopefully see you soon
What concentration of Oligomycin are you looking at in your final solution? The Merck index has solubility in water as 0.002 g/100 mL, or 20 ug/mL. Solubility in ethanol is 25g/100mL, or 250,000 ug/mL. So it would depend on what your target concentration is. You would probably be safe up to 250 ug/mL. Any pH adjustments or other buffers added to the solution would have to be factored in. Not sure what you are working on as far as analytical procedure. We worked on extraction of azithromycin from waste water using SPE and LC/MS/MS detection, if interested message me and I can provide further info. If you run into problems with recoveries (low or high) look to your vials or containers. This stuff might stick to plastic. For sample containers we used amber glass, and for standards and sample extracts used silanized glass vials with Teflon caps, through comparison studies found that this accounted for ~10% better recoveries, just by using silanized glass.
Best regards and good luck on your research -
Thank you Peter. The final concentration would be 1uM, which i think works out way low than 250ug/ml. Thank you very much for your help. Good luck
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