What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

Which drug is better to modify blood pressure in awake monkey, if I provide it in the food or drink reward?

I need to modify the blood pressure in a monkey while is awake in the recording chamber, and I am planing on provide a drug for that in the food or water used for reward... any advise? Thank you

08 February 2021 9,900 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

Experiment statistical analysis?

07 February 2021 3,635 3 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Which satellite image gives best results for Land Use land cover classification(specifically for built up regions) for Indian region ?

Landsat, sentinel and USGS satellite images have varying accuracy for identification of urban built up dynamics ? Which one is best suited for India

04 February 2021 8,632 8 View

Why do high doses of humic substances inhibit the growth of corn roots? Is there a toxicity effect?

Length, volume, surface area and dry mass of corn roots treated with humic substances increased up to 13 liters per hectare and decreased with higher doses.

02 February 2021 2,190 4 View

How can we calculate the sample size for rodents for a particular period of time?

How can we calculate the sample size for rodents for a study for one year? Are there any specific protocols to be followed for designing rodent sampling? How long should be the sampling interval...

01 March 2021 1,056 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

How do one prepare Disulfiram in olive oil solutions?

I have read several articles mentioning the preparation of the drug disulfiram (DSF) in olive oil for oral gavage of rodents. However, in those papers, there is no description of how this is...

06 January 2021 3,810 1 View

Can large samples be stored at -20 degrees prior to RNA extraction?

As part of a Covid project we are sampling urban rodents and performing RNA extractions and serology work. We have too many samples to store at -80 and have opted to perform extractions the same...

15 December 2020 5,252 2 View

The best thermal imagers in zoological research for detecting small mammals in soil?

What are the best thermal imagers to use in the study of insectivores, rodents and carnivorous mammals in soil horizons? The ability to detect herpetocomplex with a thermal imager in natural shelters?

17 November 2020 346 5 View

Reviews on Social Organization in Rodents?

Looking for papers which detail the various modes of social organization in rodent species.

10 October 2020 3,654 3 View

How can I fix our rodent's treadmill?

Our rodent'treadmill's bars show voltage but not affected animals. I cleaned all those bars as rat's urine is dielectric. Do you have any idea that helps me? Thanks

20 September 2020 5,971 2 View

Do anybody have any experience to record and analyze rodent newborn ultrasonic vocalization by Wildlife Acoustics Echo Meter Touch 2?

Hi, I would like to record and analyze ultrasonic vocalization of rodent (rat and mouse) newborn pups, and I find Wildlife Acoustics Echo Meter Touch 2 bat detector and the Kaleidoscope Lite...

18 September 2020 5,762 3 View