Hi,
I had a 3D model to study myelination in vitro. having TEM pictures of my cells being myelinated by schwann cells, I wanted to analyse my pictures, but I don't know how.
I'll be glad if you guys tell me so.
Dear Hanie, just as a quick Reply: you could try to use 'Image J' or even irfanview public accessible software...with some special parameter-functions and/or plug ins.....
Naturally there exist several other software solutions, some of them perhaps are free and without costs, some of them are available as commercial software one could find searching Internet for e. g. |digital imaging, morphometry, ultrastructural morphology, thickness calculation, nerve*(s)sterological approach |. First of all you should/could use the search function in RG-Questions and publications-archive.... Good luck and may other, knowledgable specialists chime in to your Question-thread...
Dear Hanie,
I suggest you analyze you TEMs in the same manner that others use in the remyelination field. For example, this zebrafish paper measures myelin thickness, calculates the g-ratio, and describes the how compact the myelin appears:
Article Peripheral Axons of the Adult Zebrafish Maxillary Barbel Ext...
Here are a few other papers and reviews, such as
Article Morphometric analysis of axons myelinated during adult life ...
Article Analysis of myelinated axon formation in zebrafish
Article A regenerative approach to the treatment of multiple sclerosis
These publications should give you some idea of the method of analysis.
Good Luck!
Jill
Note: For each of the papers above, I TRIED to link to the PubMed Central version of the paper, which is free to read. Unfortunately, RG automatically converted my links to ones that link to the papers archived on RG. I am NOT trying to violate publisher copyright agreements!
Jill and others....I faced the same problem for the last 2-3 weeks in other replies. Perhaps we must (should/ought to?) use another "citation technique" when trying to insert the PubMedCentral-URL for an open Access paper....
@Hanie: it would be of benefit if you let us know your test system (human, animal /species..?) if you wanted to know the "normal value" of myelin sheath thickness....Best wishes and regards, W. M.
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