How should I test technical replicates in rt-qPCR for outliers?

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Any suggestions for storage options for 10x10 1.5ml tube boxes?

My lab uses 10x10 USA scientific boxes to store plasmids and as of right now they are stacked up to 6 boxes tall. This causes a lot of box shuffling and I was looking for recommendations for how...

08 September 2022 5,973 1 View

How can you avoid partial anneals during primer design and PCR?

I designed some primers for an amplicon that is 120 bases long but I only get desired product with an additional non specific product at 550bp when using very high concentrations from 30uM-3uM....

13 July 2021 2,898 6 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 1,238 3 View

How to test multivariate outlier in STATA?

Hey all, I need help testing for multivariate outliers using STATA for my master thesis. The literature recommends the Minimum Covariance Determinant (MCD) (Verardi & Dehon, 2010). I found the...

22 July 2024 8,821 2 View

How can I prepare the csv. dataset to find the flood triggaring factor using ANN?

here's a concise guide for preparing your CSV dataset in Excel to identify flood-triggering factors using an ANN: Clean and Format:Address missing values (fill with mean/median or remove if...

21 June 2024 7,941 0 View

How to deal with zero/negative growth rates in tree growth models?

Tree growth is an important aspect of forestry and forest ecology. Typically a growth rate is calculated as the difference between two stem diameter measurements over a given time interval: (dbh2...

15 May 2024 8,739 2 View

Dealing with outliers?

Hello everyone, I am calculating the duration from Site Selection to Clinical Start Date for the process map. The duration across all groups ranges from 0 to 70 months, with mean of 5.6 months...

03 May 2024 2,624 4 View

What are "normal" PEP successive values?

Hello everyone, I work on the cardiac pre-ejection period (PEP) using Biopac ECG/ICG. I record cardiac activity on non-patient healthy adults from 18 to 65 years, during rest or cognitive and...

01 April 2024 4,702 0 View

Finding the differentially expressed genes?

1. For the gene expression data (microarray dataset which is been extracted from the Gene Expression Omnibus (GEO) platform), which of the following normalisation techniques are suggested as the...

05 March 2024 8,451 1 View

Ion ratio value in LC-MS/MS?

Hi everyone, I analyzed some samples by LC-MS/MS, and I got a pretty good peak for quantifier ion, but the peak intensity for qualifier ion is not good enough so I got "ion ratio" as outlier...

21 February 2024 4,249 12 View

Should item-level data or total scores be used for detecting multivariate outliers using Mahalanobis distance method?

I want to detect multivariate outliers in my dataset, which contains participant responses to various questionnaires such as DASS-21, PSWQ etc. Should I compute Mahalanobis distance using total...

18 January 2024 3,699 3 View

Outliers continue to appear after removal. What should I do?

Hello, I am trying to remove outliers from a dataset. I removed a few, but they continuously appear. What should I do? Please do not post AI generated answers.

16 January 2024 9,442 4 View

Can anyone suggest a good way of testing for an outliers for an experiment ran in triplicate?

I have a set of experiments ran in triplicate and I have some data points that seems like an outliers in each one? Which method is the best way to examine that? i would appreciate recommendations

21 December 2023 6,801 2 View