Ryan Scanlon

4 Questions 2 Answers 0 Followers

Questions related from Ryan Scanlon

Any suggestions for storage options for 10x10 1.5ml tube boxes?

My lab uses 10x10 USA scientific boxes to store plasmids and as of right now they are stacked up to 6 boxes tall. This causes a lot of box shuffling and I was looking for recommendations for how...

09 September 2022 5,987 1 View

How can you avoid partial anneals during primer design and PCR?

I designed some primers for an amplicon that is 120 bases long but I only get desired product with an additional non specific product at 550bp when using very high concentrations from 30uM-3uM....

14 July 2021 2,914 6 View

QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

24 February 2021 1,273 3 View

How should I test technical replicates in rt-qPCR for outliers?

I am running my plates with 4 technical replicates. How can I tests these 4 for outliers and still be MIQE compliant. I can't seem to find any published work that shows how outliers were determined.

09 February 2021 6,444 2 View