I would like to embed small pieces of plant tissue at RT in gelatin capsules with the use of LR Gold resin (Sigma), but unfortunately I have some problems with too fast polymerization. I added 0,0198 g LR White catalyst per 1 ml of the resin (according to manufacturer instruction), mixed it with the use of magnetic stirrer and filled the capsules at RT. It polymerized in about 10 min. When I reduced the catalyst concentration, upper surface was still soft, even after few hours of daylight exposure. I will appreciate any advise.
Hello Sandra,
you can either cool it down in the presence of catalyst - this should prolong polymerization time, or lower catalyst concentration and keep it to polymerize longer at RT up to 1-2 days
best,
Jozef
Dear Prof Jozef Samaj,
Thank you very much for your advise. It really helped me.
Sincerely,
Sandra
Dear Sandra!
Dr. Elliot Kitajima ([email protected]) have much experience with resins, tissue fixation and microscopy.
Sincerely,
Paulo
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