I would like to know how the IL-2, IL-6 and FAS/APO-1 regulate the auto immune diseases like rheumatoid arthritis? How could we know the toxicity level of protein using the concentration level of IL-2, IL-6 and FAS/APO-1 from T cell?
Toxicity of protein can be measured by Lymphocyte culture of both normal and diseased individuals with stimulation of your toxic substance. Then do a flow for annexine v staining. From the supernatant you can measure the required cytokines and compare.
You can see the below paper..
Arthritis Rheum. 1995 Dec;38(12):1738-43.
Lack of correlation between serum soluble Fas/APO-1 levels and autoimmune disease.
Goel N1, Ulrich DT, St Clair EW, Fleming JA, Lynch DH, Seldin MF.
Dear Prakash, Thanks for your response. If the cytokines level will increase, my protein has toxicity to T cell? Which one denotes the toxicity of protein, whether increase level or decrease level of cytokines?
Toxicity can be measure by cell death by annexine V staining flow cytometry and stimulation of your antigen can be measure by increasing /decreasing of specific cytokines.
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