I am getting Cryprosporidium parvum oocysts in rat faecal samples after experimental infection in immunosuppressed rats. I need to demonstrate the protozoan in intestinal sections as well. Since this is a superficial parasite, please suggest how should I collect intestines after necropsy for routine histopathological sectioning so as to maximize chances of demonstrating the parasite in intestinal sections.
Hudson Alves Pinto
Dear Shilpa Sood,
I think the Swiss roll technique can allow you to examine a large intestinal area and so maximize the chances of finding parasites. However, several works on histopathology of Cryptosporidium infection in rodents use the terminal 2 cm of ilea. I hope this information be useful for you.
Best regards,
Hudson
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