How remove vermiculite of root samples?

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Which is the best mitochondrial potential dye for primary cells?

Hi! I am wondering if someone can recommend me a good dye for measuring mitochondrial potential. I have used TMRE with good results in the past, but I have heard also about Mitoview. I would use...

12 January 2021 4,200 4 View

How to analyze sodium lauryl sulfate using HPLC-RID?

Hi, I am analyzing the content of anionic surfactant: sodium lauryl sulfate (SLS) in commercial detergents using HPLC-RID/refractive index detector. I used column Agilent Zorbax Eclipse XDB C-18...

15 November 2020 1,405 2 View

How do I test for gender differences in Spearman rho correlations?

I want to explore if there is a gender difference in a correlation between two ordinal items. I have calculated spearman rho within gender for the items, but I now need to test if the correlation...

09 October 2020 970 13 View

How to get rid of Matrigel during enteroid passaging?

Hello. I am trying to generate monolayers from cow enteroids. The protocol I am following involves 0.5uM EDTA and Trypsin-EDTA 0.05%, each enzyme is applied in two different steps. But for some...

10 March 2020 1,805 1 View

Is there a way to edit the citations in MENDELEY so it's in the order of citation in the text body instead of Author?

Does anyone know how to change the citation style in Mendely in order to sort the references according to the order of citation in the paper? So that in the text is displayed only the number (eg....

01 January 2020 746 2 View

What could be an explanation for making plants in their growth development appear fake growth rings?

As plants grow in their development, they undergo many changes either due to climate change or other environmental issues that affect plants but I want to know how false growth rings form and why...

16 December 2019 1,615 5 View

How to avoid impurities in immunoprecipitation with magnetic beads?

Hello, I need to extract an antigen from human serum. I tried with streptavidine-coated magnetic beads and a pair of biotinylated antibodies. Then I followed the elution procedure, as recommended...

09 September 2019 6,947 4 View

How to analyze alkyl distribution of Methyl Ester Sulfonate / Sulfonated Methyl Ester?

I need to analyze the alkyl distribution of Methyl Ester Sulfonate to know the molecular weight of my sample. I tried to use gas chromatography to do this. My method was to reflux the sample first...

14 July 2019 2,133 4 View

Hello, any suggest for a research in iot security ?

I need to define a topic,It should not be so extensive to be able to cover in a good way.My idea is to make a security comparison between two IoT technologies,however i feel the topic stills too...

12 June 2019 3,221 3 View

Can you recommend me some articles that dealing with the electronic transitions of toluene?

Hello! I'm working on assigning absorption bands to UV-vis spectra of unknown matrix. I think some are due to the presence of toluene, but I can't find any article that describes the electronic...

19 March 2019 8,119 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

Which root in this tap root system, in the picture attached, should be used for root sectioning in order to study the root anatomical details?

There are numerous roots in this tap root system. Kindly suggest or share a research paper where I can clarify the doubts as to which root should be chosen for sectioning and for further root...

14 February 2021 1,816 3 View

What is the relationship that determines the depth of the mechanical cultivator with the age or size of the weed For crops planted in lines?

the great depth of the cultivator may lead to passage under the roots without killing them and may give negative results to break up the soil below the roots, increasing their activity, especially...

03 February 2021 3,198 7 View

Why do high doses of humic substances inhibit the growth of corn roots? Is there a toxicity effect?

Length, volume, surface area and dry mass of corn roots treated with humic substances increased up to 13 liters per hectare and decreased with higher doses.

02 February 2021 2,190 4 View

Does the subsoil have an effect on the spread of weeds whose roots are more than 35 cm deep?

For weeds whose roots deepen more than the level of subsoil, they may be affected by this layer, for example, determined by height and growth, as well as limited to flower production and seeds.

01 February 2021 1,940 4 View

What is the magnitude of yearly rhizopdeposition in tropical forests?

Hi all, I am trying to find reference values on annual soil rhizodeposition rates for different kinds of forests, with a particular interest for tropical rainforests. Do we have an idea of the...

26 January 2021 4,278 4 View

How to use plant root tissue for experimental analysis?

Hello everyone, I am working basically on plants (hydroponic culture). I want to estimate biochemicals and gene expression pattern of root tissues. My question is that how to harvest the tissues...

20 January 2021 4,443 3 View

How to Extract DNA from dried rhizomes, wood, galls etc. stored for a long time?

Dear Respectable Scientists, I have been trying to extract DNA from above mentioned sources using different CTAB/SDS Buffer Compositions and purifying agents but I am not able to extract DNA from...

12 January 2021 5,899 8 View