Mid log phase is a point between the end of lag phase and the start of stationary phase. It can be calculated from preliminary experiment data, and will depend on medium composition, inoculum type and amount, strain, volume of the medium, intensity of aeration (rotation /shaking rate), and temperature. Most usual 1/2 of log phase time is observed between 8 and 24 h of cultivation.
it is true that the availability of nutrition, temperature, strain , amount of inoculum and aeration rate affected the Cultivation of microorganisms. Adaptation of the strain to the minimal medium could be shortens the time of observation. Agree with Alex Ignatov
I agree with Alex Ignatov as seconded by Tarzan Sembiring; do a few preliminary experiments (you can vary inoculum amounts and other variables based on your specific requirement of course) and what your answer is.
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