Typically, flash chromatography purification of 1g of sample will require 20 g to 100 g of silica, and 200 to 1000mL of total elutant volume. If the column is successful, the desired component should be eluted in no more than 1/10 of the total elutant volume.

Go through this for further details

https://reachdevices.com/SetUpColumn.html

Kashif Ameer Thank you Dr. Kashif, this is the amount for the column itself what about the preparation of the sample if the sample is not liquid we should add solvent and silica to the sample first, how much should we add?

Ahmed Amin As your sample is only 300 mg so you may add merely 50 g to the sample first and then go ahead

Kashif Ameer Thank you Dr.Kashif

Ahmed Amin Welcome