Greer Laboratories offered me lyophilized HDM extracts indicating the Derp1 in mcg/vial and total protein in the vial. People in the papers write that they use e.g. 100 micro-gram HDM in CFS. Does it 100 micro-gram Derp1 or 100 micro-gram protein? How much CFA(ul?)+100 ug HDM shell I use per mouse for the s.c. sensitization? What is the ration? Greer offers endotoxin containing and endotoxin free HDM extract. Which is the best for steroid insensitive model?

Do you have any idea how people add intranasaly/intratracheal water insoluble budesonide or fluticasone in liquid formulation?