What are you doing with the extracts? Biological assays can tolerate some DMSO, but are limited to a certain percentage, 10% or less, depending on the assay.
Chromatography can often tolerate more DMSO but a large volume of DMSO can affect the chromatography. For example, DMSO is a "weak" solvent with C18, but is is "stronger" than water, and reduces the ability of polar compounds to interact with the column.
i want to check the antifungal activity of these extracts in plants . i made extracts in 1:10 ,I GRAM Of plants extracts dissolve in 10 ml of ethanol nd dried them now store in 4c .how can i dilute my extracts? nd 10 % dmso means 5 gram of plants extracts dissloved in 95 ml of solvent (dmso ) ?
By 10% DMSO, I mean that the extract is dissolved in a solution containing up to 10% DMSO, the remainder being water.
For following bacterial or fungal activity, one can dissolve the extract in a solvent, and apply the solution to paper antibiotic disks, and evaporate the solvent. Put the disks on the agar and incubate. See the link below for an example of the disks.
One can also do TLC on the extract, and do bioautograms. Run the TLC, evaporate the solvent, lay the plate down so the media is on the agar, and let it sit for an hour or so. Remove the TLC plate, incubate. One can then see if the antibiotic moves with that TLC plate and solvent system.
Here is one example of paper antibiotic disks: http://www.carolina.com/catalog/detail.jsp?prodId=805091&s_cid=ppc_products&utm_source=google&utm_medium=cpc&s_cid=ppc_gl_products&scid=scplp8194010&sc_intid=805091&gclid=CKje6tLZ8M4CFZYjgQodISUKVg )
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