it depends upon the used instruments and the method of analysis but it is better to draw blood as mush as you can even 5 ml to be in the safe

side

approx 5-6 ml is sufficient for AO activity by spectrophotometer. firstly you should separate the serum from blood.

For these parameters you would need blood serum and you can use 25ul to 100ul for each parameter (you would need in triplicates at least). So you can perform 3-4 parameters from one rat.

4-5 ml blood (1-2 ml serum)

Its depends upon the reaction mixture and method of analysis.

If you are using micro plate assay less than 1 ml serum is sufficient. If not 1-2ml serum

Approximately 5 ml

At least 5 ml blood is required to separate 1-2 ml serum

this is determined by the technique you are using. There is a macro- and micro-definition for each indicator

2-3ml blood from which we can get 1-1.5ml Serum

Blood must be taken from the abdominal aorta. For the study, 1-2 ml of serum (sometimes heparinized plasma) is necessary. Studies are also conducted in hemolyzed erythrocytes.

2ml serum should be enough

The most informative is the determination of markers of oxidative stress and AO-system in the cytosol and mitochondria of various tissues of the target organ

Approximately 2-3 ml blood from which we can get 1-2 ml serum.