I am using 5 FU liposomes for transdermal delivery but I am confused a little bit regarding how much formulation must be loaded into donor compartment of the Franz Diffusion Cells for 5 FU liposomes which must be cytotoxic to cancerous cells. I browsed the internet for weeks but didn't find a satisfactory answer since all topical available formulations of 5 FU state to "apply twice a day". But no answer to the question of exactly how much 5 FU must be applied.
You can just put 1 ml in donor compartment and form your liposome study you know how much drug is there in donor compartment. Because in the end you are going to measure the concentration in receiving media which should be negligible and at the end of experiment you can perform mass balance experiment to find out actual amount of drug.
At lease 10 ul/cm2. You can use more to have longer steady-state phase to be able to calculate the permeation flux. I recommend 100ul/cm2 or more. Low doses are closer to clinical conditions but might not allow you to calculate the flux properly.
Good Luck
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