Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

Explain plasmid description?

Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....

14 January 2021 6,326 2 View

How big the Balb/c mice spleen after immunization?

I am working with Balb/c mice, and in my experiment, I have to inject mice with antigens, then get their spleen and collect the splenocytes for fusion. Recently, I get into trouble with the mouse...

04 January 2021 3,591 3 View

Capture of oleophilic solid polymers present in water solution ?

I am looking for articles, coferences, publications or thesis concerning the capture of solid oleophilic polymers in solution in water. Thanks Thierry Le Blan

01 January 2021 5,798 3 View

Can I do gene expression with partial cds (not the complete one)?

Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...

27 December 2020 3,688 3 View

Hybridoma stops producing antibodies after thawing from liquid nitrogen?

Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...

01 December 2020 1,178 3 View

Suggestion for normal ovary cell lines?

Dear all, I want to choose a normal ovary cell line and check for its proliferation property when introducing carcinogenic factors. Does anyone have any suggestions for the cell line? Many thanks...

08 November 2020 9,100 3 View

What is Snapback Primer in realtime PCR?

Dear all, I find a paper using Snapback primer. I do not understand the function of snapback tail? What is snapback primer? When do we use snapback primer? and why we use that? Thank you.

01 November 2020 8,869 2 View

Rotation matrix - 3D dimension to 2D dimension?

22 October 2020 9,354 4 View

Am I introducing any potential loss of RNA integrity by combining an RNALater solution with a non-ionic detergent?

I am planning to characterise the transcriptome of pre-anthesis kiwifruit anthers. The samples are being harvested on my behalf and sent down by post, and I cannot ask the harvesters to dissect...

10 October 2020 1,830 3 View

Which factors contribute to instructional resilience in HE?

If you are teaching in Higher Education, please consider participating in our study on Instructional Resilience during Emergency Remote Teaching. We want to learn about which factors have...

19 January 2021 6,759 15 View

Can the benefits of resilience be linked to the types of resilience?

Hi All I am trying to obtain journal articles on the benefits on resilience. However, I was thinking - can the types of resilience (physical, emotional, psychological & community...

02 January 2021 7,409 6 View

Can you help me find a conference in field of urban disaster recovery?

Dear Researchers. I am looking forward to finding a well-known or prominent conference that has proceedings in disaster recovery, urban resiliency, sustainable development, and community...

01 November 2020 8,666 2 View

Is there any good psychological work or intervention that can reduce fear of COVID-19?

Hello, everyone! I would like to work with Japanese people, mainly Japanese university students, to reduce their fear of coronavirus and their anxiety about the future. If anyone knows of a work...

22 October 2020 1,905 65 View

Does anyone know about intrument for academic stress during distance learning in pandemic?

my research is about relationship between resilience and academic stress during distance learning. I really apreciate if you answer my question. thanks before

03 October 2020 6,305 4 View

What can be an alternative use for a survey that measures resilience that is valid and reliable??

I really need help in my undergraduate research because I am following a timetable. I just need to assess the level of resilience of selected nurses in the local setting. P.S. As an...

30 September 2020 4,740 10 View

Do I have to run a factor analysis on a measure that is already validated?

I am using a 33-factor resilience scale that has been consolidated into 6 factors and validated several times in the past 15 years. Do I also have to run a factor analysis if I am using the...

15 September 2020 5,184 4 View

What type of regression can I use to analyze a continuous dependent variable with multiple categorical independent variables (ordinal)?

I have a dependent variable (resilience scores) that was devised by summing Likert scale (1-5) responses. I was advised to consider this as a continuous dependent variable. I have 6 independent...

25 July 2020 8,037 2 View

Why do some informal settlements and societies successfully adjust and even thrive amid adversity while others fail to do so?

Many informal settlements have insufficient capacity to forecast, check, handle and reduce disaster risk. These communities face a growing range of challenges including economic hardship,...

23 July 2020 3,511 8 View

What emotional intelligence theory is best for university academics?

Hi Please advise. If looking at the relationship between emotional intelligence and resilience in university academics - what would be the best EI theory to use? Considering we have ability...

09 June 2020 995 5 View