Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View

Dataset size and the need for cross-validation (medical domain)?

I am currently envisioning a deep-learning model that classifies pulse waveforms (normal vs. pathologic). At this time, the no. of pulse dataset is about 2,000,000, and the balance of normal and...

31 July 2020 6,713 3 View

What will be coefficient of friction for mild steel tube when axially impacted by a mild steel projectile ?

i am doing a study related to axial compression on mild steel .i need coefficient of friction value when mild steel tube axially compressed by a large mild steel projectile.i am using abaqus FE...

13 April 2020 259 4 View

What is the fracture energy of mild steel ? how can get from true stress and true strain graph?

I am using abaqus /explict for modeling with j c model for target material is of mild steel and i required fracture energy in in damage model

05 March 2020 4,241 2 View

What is the mass of CO2 at 1 atm pressure and RT ?

I want to calculate the Sheldon E factor for (environmental and sustainability) of reaction. In my reaction i have used 1 atm CO2 as one of the reagent so we need the amount of CO2 in (gm/ mg)...

30 July 2018 7,927 2 View

How can i use EMBL pride inspector?

Hellow everyone. I am supposed to use EMBL pride proteomics documents. But I don't know how to use EMBL pride inspector program. even there is no file to introduce inspector program. Up to now, I...

29 May 2018 4,754 2 View

Has anybody used the user subroutine for single crystal plasticity provided by Prof. Kysar (originally by Dr. Huang) for studying texture evolution?

I am using the subroutine for polycrystal plasticity but I am not able to obtain right deformation texture. if anybody has already used it for the said purpose, would be delighted to seek guidance...

13 March 2017 8,400 3 View

How to stain macrophage, especially mouse macrophage j774a, in relation to immunohistochemistry?

16 October 2016 8,317 2 View

Isn't there any other way to collect a flagella of salmonella typhimurium?

09 June 2016 1,452 3 View

Any advice on the transfection reagent for mouse ES cells?

Hey guys, I have try several transfection reagent (Xfect, lipofectamine 3000, PolyJet) for transfecting mouse ES cells. But the transfection efficiency is quite low. The best I can get is 50%...

23 October 2015 5,369 9 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

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01 March 2021 3,905 5 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

Best excel chart to compare algorithms' running time?

Hi I want to compare the running time of robotic path planning algorithms. The grid size is 8X8. I have 100 scenarios of start and goal positions.. Each scenario is run in three obstacles ratio...

22 February 2021 621 1 View

What is recommended and opened real-time or nearly real time geospatial data used in flood risk assessment and regional planning on a detailed scale?

I asked recommended real-time or nearly real-time data source for any parameters for flood risk on a microscale analysis for urban area cases in developing countries that had agglomeration urban...

18 February 2021 2,617 2 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View