How many intrusion responses have been developed in cloud and mobile cloud technology in 2013 and 2014?

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Lentivirus transduction of HEK293T cells?

i need to ask that when I transduce my HEK293T cells with Lentivirus, how much time will they require for transduction because when I add lentivirus and then change the media after 24 hours, my...

07 July 2024 2,946 1 View

Over expression in HEK293T cells?

Hello researchers, i need some help, i am packaging Lentivirus( having my transgene) in HEK293T cells, as I will collect the supernatant and concentrate my lentivirus,I also want to check...

15 June 2024 1,596 3 View

Lentivirus and HEK293T cells?

i need some help setting up my culture plate having negative controls,I am going to infect HEK293T cells in a 6-well plate... to one well I will add my lentivirus particles(packaged already), and...

15 April 2024 7,218 5 View

Cytokines production in HEK293T cells?

Hello dear researchers,i have transduced my HEK293T cells with IL-2 and then puromycin screening was done, the cells were showing good egfp flourescence ,but then due to some reasons I have to...

14 April 2024 7,285 2 View

Effect size indices and 95%CI could be presented?

I received comments from a reviewer on my manuscript having RCT design and I compared pre and post data by pair t- test. The reviewer commented: Effect size indices and 95%CI could be...

26 January 2024 1,980 2 View

T lymphocytes isolation?

Hello to all of the researchers, i need to isolate and grow T lymphocytes from swine lymph nodes. I need you people to guide/help me or send me any protocol/video for T cells isolation from wine...

24 January 2024 1,380 1 View

HEK293T cells culture?

Hello dear researchers, i am generating a stable cell line of HEK293T cells using lentivirus, i want to ask about the puromycin kill curve,if i seed my cells in a 12-well plate on day 1, Q1) when...

04 January 2024 3,109 4 View

HEK293T cells culture?

Hello, i have grown my HEK293T cells in 4 wells of a 24-well plate and added different serial dilutions of lentivirus(containing my gene and GFP), now i want to perform FACS analysis so for this...

30 November 2023 8,866 2 View

Lentivirus titration for infecting my target cell line HEK293T cells?

Hello to all researchers, i need to do lentivirus titration and for this I need 10 fold serial dilution i.e 10^-1 to 10^-4...and i have my lentivirus stock stored with me...second thing is if i...

28 November 2023 9,880 1 View

Lentivirus packaging for cells transfection?

i have my gene of interest in this way(picture in attached files), Now I am confuse about whether i can use this whole thing to construct my one the 3 lentivirus packaging vector named pLenti...

03 September 2023 7,906 3 View

Stability of the Solar System: Insights from Einstein’s Equations ?

The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...

07 August 2024 2,569 1 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,492 0 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

Which test should be used to study association among demographic profile and awarness level?

i have to study the awareness and adoption level of cloud computing in a district of India. i also want to use association among demographic variables like gender, age, education, income etc and...

02 August 2024 2,420 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

How to Compress Information Neurally?

Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...

01 August 2024 4,456 2 View

Can I proceed with Response Surface Methodology (RSM) with only one factor for further optimization?

I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...

29 July 2024 5,015 1 View

How to use Desmond in HPC ?

Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...

28 July 2024 6,553 1 View