Yield of the Lipopeptide from Bacillus sp., i am getting more when i am using LB broth as a production medium.While in the case of Nutrient broth and potato dextrose broth, yield is less. How can i justify this ?.
LB broth contains tryptone (a mixture of peptides formed by the digestion of casein with the pancreatic enzyme, trypsin), yeast extract and NaCl.
So tryptone as it is peptide may enhance the prodcution of lipopeptide from Bacillus sp. in your case as it may be suitable substrate for bacteria.
Microbial culture media can be of different type, depending on the nutritional growth requirements of the microorganisms. Microorganisms require about 10 macroelements namely (C, O, H, N, S, P, K, Ca, Mg and Fe). The first six components are used in the synthesis of Carbohydrates, Lipids, Proteins and Nucleic acids and the remaining four exist in the cell as cations and play a variety of roles. In addition to macroelements, all microorganisms require several microelements like (Mn, Zn, Co, Mo, Ni and Cu). These are generally part of enzymes and cofactors. Microorganisms also require growth factors, which are organic compounds.
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