We have isolated ANG (1-7) in a C18 column through solid phase extraction. Elution has been done with 5% Formic Acid in MeOH, but we are experiencing drying times in excess of three hours. Is there any way we can speed this process up?
It should take, "as long as it takes". Depending on the method used and material, some volumes will take longer than others. How to speed up the process? For most types of solvent extraction in the laboratory, we use a rotary evaporator to speed up the process (vs drying under nitrogen gas in the fume hood). A rotary evaporator should be set up at an appropriate temperature, using glassware sized for the application. Training should be obtained to use the device and most schools will have manuals or guides to assist you in this process.
Here is one example of such a s "guide" available on the web (*Please refer to the operator's manual of your 'rotovap for more information)':
https://chem.libretexts.org/Bookshelves/Organic_Chemistry/Book%3A_Organic_Chemistry_Lab_Techniques_(Nichols)/05%3A_Distillation/5.05%3A_Rotary_Evaporation/5.5.0B%3A_5.5B%3A_Step-by-Step_Procedures_for_Rotary_Evaporation
(You provided no information on the volume OR the method of solvent removal used, so no specific answer to your specific question is possible. When you have a question, try to be specific and include details in your request. This will help others to help you). Please consult your instructor for help and/or anyone familiar with laboratory solvent removal techniques.
It should take, "as long as it takes". Depending on the method used and material, some volumes will take longer than others. How to speed up the process? For most types of solvent extraction in the laboratory, we use a rotary evaporator to speed up the process (vs drying under nitrogen gas in the fume hood). A rotary evaporator should be set up at an appropriate temperature, using glassware sized for the application. Training should be obtained to use the device and most schools will have manuals or guides to assist you in this process.
Here is one example of such a s "guide" available on the web (*Please refer to the operator's manual of your 'rotovap for more information)':
https://chem.libretexts.org/Bookshelves/Organic_Chemistry/Book%3A_Organic_Chemistry_Lab_Techniques_(Nichols)/05%3A_Distillation/5.05%3A_Rotary_Evaporation/5.5.0B%3A_5.5B%3A_Step-by-Step_Procedures_for_Rotary_Evaporation
(You provided no information on the volume OR the method of solvent removal used, so no specific answer to your specific question is possible. When you have a question, try to be specific and include details in your request. This will help others to help you). Please consult your instructor for help and/or anyone familiar with laboratory solvent removal techniques.
The dependent factors are multifactorial, and include:
Total volume of solvent
% dissolved solids
Sample format of the solvent
Equipment used for drying
Settings used for that equipment
Answer some of those, and happy to help try and answer your question.
if you add some heating (35 to 45 celsius) could help
Best regards
Gustavo Monnerat
Hi Kaden,
All the respondent make good points. In addition, the type of solvent really matters. Heavier solvent do take more time than the lighter ones. So, warming the mixture up and increasing the pumping pressure if possible will be a great option.
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