Hello. What would be the recommended duration for the usage of a Matrigel + DMEM-F12 dilution? One or two weeks?
I use a "thin" matrigel coating for iPSCs - wherein I dilute about 500 ul of a matrigel aliquot in 25 ml DMEM-F12. I'm not aware of the protein concentration of the matrigel, I've been using aliquots from a colleague who follows this procedure.
Thanks!
Hi,
6 well plates covered with diluted Matrigel (wrapped in parafilm) should be stored in the refrigerator for up to 2 weeks. The DMEMF12+Matrigel in Falcon solution can be stored in the refrigerator for up to 2 weeks. Generally, the DMEMF12+Matrigel in Falcon solution can be used multiple times until the cells detach from the plates. Multiple uses of this solution do not affect the characteristics of the iPSCs.
Best,
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