I’m using saliva sample of cancer patients for the metabolomic profiling to biomarker discovery. In connection with that I have some doubt in storage of saliva sample.
1. I want to know that the how long I can store the whole saliva in -80oC for the untargeted metabolomic study using LC-MS method?
2. Is the saliva more stable as whole ?
3. After sample preparation for the metabolomic studies how long it can be store in -80oC?
Metabolome is always prone to degrade overtime if you repeatedly go through freeze and thawing. However, if you don't do that, it should be reliable.
To check how much or who are being degraded due to freeze and thaw, you may profile same samples after 1, 10, 20, 30 times freeze and thawing. If you find any peak got problem, you may keep them aside for down-stream analysis. These are sensitive species, so your longtime stored samples can get affected. So, you may keep aside them always. Good luck!
@Md Saifur Rahman Khan I'm having the same problem..
But instead of saliva, I have cancer tissue sample for gcms studies. I about to do this in a week.... The processing of whole tissue sample for gcms will probably take a day.. so, my problem is "gcms technician guy will not be available after 5pm of working day. So he asks me to do in the next day morning? So if freezing the sample for half night, will it affect my peak? Or suggest me any optional strategy for that..
Any comments, I hope to hear from you...
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