How long dried plant virus-infected leaves remain valid for viral RNA extraction efficiency?

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Gloria Ceasar

they can remain valid for very long as long as the leaves are kept in properly i.e away from dump conditions which can cause fungal growth. we work on cassava viruses, we keep our samples (leaves) in magazine print papers which allow air to pass through and also does not retain moisture. our samples remain in good conditions for very long.

Houda Kawas

Dear Gloria Ceasar

Thank you for your answer,

I have infected dried samples more than 25 years (for PLRV, BWYV..) I want to compare the isolates with new one by Rt-PCR, its work with you for this long time, not for mechanical transmission.

Best regards

Houda

Sandeep RAJ R

it will be alive and well as ur viruse are RANA viruses unlike ours whose mRNA degrade in matter of day when kept outside, viruses basic stucture is a lot their genetic material hence it is made tuff and resistant to degradation, best of luck for ur real time work...

Dear Sandeep RAJ R

Thanks a lot