Hi, i wanted to know if it is possible to perform ng genome sequencing of a vertebrate species within a year? starting at almost zero previous knowledge
as warned previously, I would say what is your other experiences, just want to train or seek advice of others, than a rough draft can be ready in the year time, it requires time!
The molecular techniques involved in preparing DNA for sequencing are not terribly difficult, depending on your current familiarity, and can also be performed by the sequencing core facility for relatively little compared to the actual sequencing cost. What is often the hiccup for people is the bioinformatics end. You will need familiarity with Linux/UNIX, (probably) some sort of access to a high-performance computing platform (or a very beefy workstation), at least intermediate knowledge of some form of scripting language like Python or Perl (IMO- maybe you can get by without it), and on top of that an understanding of the vast array of software pipelines and tools available for de novo assembly.
Since the steepest learning curve is on the bioinformatics end, I would recommend starting with downloading raw data from available archives (e.g. from NCBI short read archive, or Data Dryad) or the many tutorials online, for a small genome (maybe some bacteria), and work on figuring out the most common tools for quality control of your data, assembly, and scaffolding.
That being said, a vertebrate assembly in a year might be pretty difficult. It depends on what you are looking for in an end product- a highly fragmentary draft assembly can be built very quickly, but if you are looking for a more complete assembly, with functional annotation... That's a lot of work even for a group with experience