I am trying to inoculate healthy potatoes with PLRV and PVY using Myzus persicae as vector.
I came across a question where if I could let aphids obtain the viruses from excised leaflet by letting them feed on the leaves for their own appropriate acquisition time.
Can I create a leaf disk using those leaves, petri dish and filter paper and place aphids that went through starving period to make the aphids obtain transmissible viruses?
This should be no problem at all with PVY whose longevity in vitro is reported to be 7-50 days in crude plant sap (and surely even longer in leaf disks) at 25°C. PLRV is by far less stable but should also remain infective during the aphids' feeding / acquisition period in leaf disks.
Dear Jung-Wook Kho
Longevity in vitro differ from virus to another
Yes you can, you may also try membrane feeding
you may visit my researchgate
Regards
Prof. Houda Kawas
Thank both of you for your kind answers!
It's been a great help.
Have a great day!!
You are very welcome. You might also want to refer to http://dpvweb.net/dpv/showdpv.php?dpvno=291
Good luck with your experiments.
Sandra
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