2.6 Drying gas flow controller cannot regulate the requested setting after a fixed amount of time?

We keep getting this error on our Agilent 6470 TQ. We were told it could either be our N2 generator or filter traps. We've called the manufacturer of our generator and they believe it's working...

21 January 2021 2,969 1 View

TRAP staining (rat femur) faids away ovenight?

Hi, does anyone know why TRAP staining faids in FFPE rat femur sections? My protocol: After deparaffination and rehydration i put the slides in Acetat Buffer for 20min, then they go to the...

14 December 2020 3,112 4 View

Can anyone help me regarding the doubling time of JAWS II cells?

Anyone with experience in growing JAWS II cells could give me an idea of its doubling time? I intend to use these cells for transfection protocols. Any tips to help with growing and transfecting...

01 December 2020 855 3 View

Which software, other than NVivo, do you use to code and perform thematic analysis of your interviews?

We undergone so much problems with NVivo (activation, compatibility PC vs MAC) and it is so much expensive.

07 July 2020 8,222 10 View

Secondary structure causing late amplification in NTC of TaqMan qPCR?

Hello, Excluding contamination and probe degradation, can anyone suggest a cause of late amplification in the NTC of TaqMan qPCR ? I considered the possibility of primer dimers, but since they...

21 March 2020 8,904 3 View

What can I do to improve my 260/230 values?

I am experiencing low 260/230 values after using Qiagen's DNeasy PowerSoil Pro Kit. I have been getting values anywhere from around 0.11 to 1.10. After speaking with a Qiagen rep, she recommended...

03 March 2020 4,203 7 View

Can 4% PFA in methanol free PBS fix AND permeabilize cells ?

Dears, I used 4% PFA to stain in vitro cultured cells for protein staining (Cristal Violet). No I would like to stain lipid droplets in the cell with oil-red solution. I thought I will NEED...

06 February 2020 2,010 1 View

Doubt about anti-mouse IgG-CFL 488 sc-362259 specificity?

I am using the rabbit anti-mouse IgG-CFL 488 sc-362259 from Santa Cruz and because some problems we performed a test using the secondary antibody without the primary and we realized this antibody...

15 January 2020 2,947 1 View

Is there any maximum number for studies that can be included in meta-analysis?

I'm doing a meta-analysis under a broad topic and about 50 studied reached my inclusion criteria. Is that number acceptable and if not how many studies should I have? Indeed, the topic of this...

12 September 2019 1,644 4 View

C-myc negative control cell line?

Hello fellows, I am looking for a cell line or cell type, which shows no or very low expression of c-myc. I want to use it as a negative control in PCR. Has anybody checked the RNA expression of...

28 July 2019 3,691 3 View

Is it possible to use EEGLAB (matlab toolkit) to extract features?

In my Final year project, I'm working on EEG emotion detection. I want to extract features from DEAP DATASET to detect emotions(happy, sad, depress etc.). I'm using EEGLAB for preprocessing, it...

23 October 2020 569 3 View

Nothing on the plot in EEGLab- what could be the reason?

Hello, I have 20 channels EEG data in txt file. I imported it to Matlab and saved as a numeric matrix. When I import it into EEGLab and open a scroll plot there's nothing on the plot. I attach a...

08 June 2020 3,207 9 View

Hello, can any one help me with reading EEG signal ?

The EEG signals stored in .npy, i read it as numpy array, but i cannot visualize it neither using mne library in python nor eeglab in matlab(if i store it as txt file)

18 October 2019 5,880 2 View

Is there any way to mark bad sections in continuous data in EEGLab WITHOUT deleting those sections?

Hello! I am currently building a new pipeline for EEG data analysis within EEGLab, and in the past I've had undergraduate research assistants do a first pass at visually cleaning the data to...

08 September 2019 945 2 View

How to get event information to load in EEGLAB?

When I'm importing my BioSemi EEG files into EEGLAB, some of them give the number of events. However, a number of files show the events as "none". Why is it that the events are showing for some...

07 August 2019 505 1 View

How to read peaks in ERP epochs?

Can somebody teach how to read peaks in ERP in order to observe peak amplitude and latency measurments in ERPLAB. I am unable to understand why in this figure No positive peak(P100) was found in...

26 July 2019 1,160 3 View

Which EEG connectivity plugin (or third-party) is best compatible with EEGLAB?

And, in addition, yields the best visualization? Statistics do not matter as they are usually computed through R, anyway. I'm already familiar with SIFT and EEGNET. All kinds of...

17 June 2019 9,986 4 View

How can I create a new dataset from the subtract of two datasets in EEGLAB?

17 June 2019 4,532 3 View

Which EEG connectivity plugin (or third-party) is best compatible with EEGLAB?

And, in addition, yields the best visualization? Statistics do not matter as they are usually computed through R, anyway. I'm already familiar with SIFT and EEGNET. All kinds of...

05 June 2019 8,248 4 View

How can I create a new dataset from the subtract of two datasets in EEGLAB?

05 June 2019 858 2 View