For culturing Mycobacterium tuberculosis strains, we use 7H9 media supplemented with 10%OADC. Once prepared separately, how long can these be stored before use? Also, what might be the proper storage conditions? Is it advisable to mix 7H9 and OADC (without any antibiotics) to make a stock, and aliquot from it as and when needed ?
If you mix the 7H9 with the OADC (or anything with any protein), the rule is almost always 1 month at 4C. (But I have used the mixture up to 6 months at 4C that it still work.)
Separately, the 7H9 can be stored at 4C for probably up to 1 year (but I don't think I ever gone more than 6 months).
OADC is usually at 1 month at 4C. I guess you can freeze aliquots at -20C for up to 1 year. The premade OADC expiration date is usually 1 year (I believe) when stored frozen.
I don't think it is recommended to freeze media.
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