Is it possible(or logical) to notice an elevated autophagy using LC3-II based immunoblotting, but no difference by using GFP-LC3 based imaging study?

For example, I treat cells with "X" - performed immunoblotting for LC3-II - found elevated autophagy, then did the same treatment in GFP-LC3 transfected cells followed by confocal imaging - found...

06 April 2020 4,332 4 View

Any Suggestions to rectify negative intercept in HPLC?

Drug is Sodium citrate, diluting drug in de-ionized water. Using a C18 column. the concentration range of 1000-3000microgram to lower range of 25-300microgram. Thanks!

19 September 2019 7,315 3 View

Can any one help to get complete bandwidth from 2 to 12 GHz which was mentioned in this paper using HFSS?

can any one help to get complete from 2 to 12 GHz bandwidth which was mentioned in this paper using HFSS... I got 5 to 12 GHz bandwidth Paper Title: Small Square Monopole Antenna With Inverted...

17 September 2019 3,508 3 View

What would be the mobile phase for quantifying sodium citrate using HPLC method?

I have to determine concentration of sodium citrate using HPLC method. Previous literature's used potassium phosphate buffer of pH 2.5 as mobile phase (isocratic). As there is risk of clogging the...

31 July 2019 1,101 5 View

Do we have a specific model and strategy for industry-university collaboration on autonomous vehicle products and services?

The challenge faced by the autonomous vehicle industry is to keep pace with the exploration of autonomous vehicles. The industrial research centers are limited and prevent them from sharing their...

08 June 2019 5,916 3 View

Why wavelength 546nm (UV) is chosen to check incompatibility of 2 drug solutions?

Looking for Turbidity of a solution defined as an absorbance value >0.015OD, which is measured using UV equipment. Wavelength used in most of the incompatibility papers is 546nm, irrespective...

30 May 2019 5,737 3 View

What would be a good brief food frequency questionnaire for use in parents of elementary school aged children?

I understand from the literature that child reports are more accurate than parent reports for school children, but wanted to specifically look at a brief ffq of parents for the same .

04 April 2019 8,643 1 View

How to validate the researcher as an instrument in qualitative research?

I am post doctoral student conducting a qualitative research. One of my thesis correction requirement was for me to state how I validate the researcher as an instrument since the researcher is...

24 December 2018 787 3 View

Example survey question for measuring efficiency in textile production unit?

creating a survey questionnaire for my research project.

02 September 2018 3,352 1 View

What is the detailed procedure for brush coating of commercial and synthesized Graphene oxide on Gas Diffusion Layer for ORR in fuel cells?

I have tried several times brush coated the commercial graphene oxide (CGO) on Gas diffusion layer (GDL) but every time the coating forms thin stripes (like zeebra stripes) and not at all...

28 February 2018 9,639 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Fractional impervious surface (FIS) map ?

Need Help Does anyone Know how to make a Fractional impervious surface (FIS) map from NDVI map, in ArcGIS environment? Thanks in advance

24 February 2021 8,455 2 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

What is the most important parameter of a research work, the impact factor or the h index?

The impact factor is a measure of the importance of a journal based on the number of citations received in a year. ... Articles published in a journal with a high impact factor do not all receive...

24 February 2021 7,481 2 View

How to calculate refractive index (n and kvalue) against energy (in eV) data for alloyed nanomaterials through Independent Phase Approximation method?

Suggest me the method or some other software to calculate the above said data for nanoparticles.

21 February 2021 3,350 7 View

How to overcome convergence issues in concrete arch models?

Hi, I have modelled a three-span arch bridge with soil fill over along with pedestals. The FE modelling is done in LUSAS with HX8M solid elements (i.e. 3D solid modelling). The non-linearity is...

20 February 2021 1,346 2 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View