I used vermiculite as a medium for soybean roots and then apply SA solution to the roots for to the check the effect of exogenous SA on the expression pattern of some genes using qRT-PCR. I repeated the experiment several times but the results are contradictory everytime. I am planning to use hydroponic solution instead, to ensure the uniform distribution of SA to the whole root. it will also allow the uniform distribution of rhizobia to the root, I guess. I just want a suggestion from someone who has already worked with a hydroponic solution to suggest the best nutrient solution for soybean roots and also the frequency at which the nutrient should be added regularly.
Thanks in anticipation
Relationships with beneficial microbe populations in the rhizosphere occur in hydroponics just as they do out in the field, with diverse and beneficial microbe species found in a wide range of different soilless systems. Certain exudates released by roots are used by plants to attract and select certain micro-organisms in the rhizosphere. These microbes can then work, via different mechanisms, to influence plant health and growth. For example, root exudates act as signals that encourage and initiate a relationship or symbiosis with rhizobia and mycorrhizal fungi, as well as rhizo-bacteria, which is beneficial for both microbes and plants.We have yet to fully understand such responses.
Nice feedback Dr Malhotra. Use of rhizobacteria in hydroponics could trigger better opportunities to go through the plant tissues as endophytes. I think, hydroponics offers a much better possibilities to watch out the competent endophytes but again keep pH and EC uniform under hydroponics is again a different challenge to address at..
Thank you so much Malhotra for your deep insight about the topic. I will really appreciate if you can please share something about the composition of hydroponic solution keeping in view the N2/nodulation relationship. I will also be glad if you can please share something about the change of solution? I mean whether we have to change the whole solution after three or four days or we simply add nutrients in the form of stock solution? Thanks in anticipation
Thank you, Parnoshree Dey, Well that's ok if SA declines as I a just need to feed the roots once for SA. I am much interested in the nutrients decline and PH-maintenance. I will appreciate if you have any idea for that and if you can please share
We applied SA to hydroponically grown roses in perlite, the concentration of SA decreased by time. Furthermore it makes the solution more acidic which you should consider for hydroponic culture.
The best
THank you, Sasan. Can you please share the detailed protocol you used?
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