The process of how lignin is determined in a wood sample in the laboratory.
There are number of options with slightly different output: briefly the the simplest way you hydrolyzed carbohydrates lignin will stay solid (klasson) and just partially dissolved (acid soluble). Have a look https://www.google.se/url?sa=t&source=web&rct=j&url=https://www.nrel.gov/docs/gen/fy13/42618.pdf&ved=0ahUKEwjk0tuq_N7ZAhWrh6YKHWESCn4QFggmMAE&usg=AOvVaw1lMHD7gZwkwJezXUoDhrwv
Another more advance is https://www.jove.com/video/1745/comprehensive-compositional-analysis-plant-cell-walls-lignocellulosic
Determination of Structural
Carbohydrates and Lignin in
Biomass Laboratory Analytical Procedure 2008 NREL; both open access
Dear Benjmain Appiah. The content of lignin in wood and other vegetable raw materials is predominantly determined by direct methods. They are based on quantitative isolation of lignin by removal of extractive substances by appropriate extraction and polysaccharides by hydrolysis with concentrated mineral acids. From an analytical point of view, lignin is understood to mean that part of the dehydrated plant material that remains in the form of an insoluble residue after hydrolysis of holocellulose polysaccharides or when removed indirectly by removing the cellulose.
There are many methods to estimate lignin content in wood sample. The method given in- Determination of Structural Carbohydrates and Lignin in Biomass. Laboratory Analytical Procedure 2008 NREL, US D.O.E. (Sluiter et al 2008) -is quite good. You can estimate precisely the contents of acid soluble and acid insoluble lignins
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