I need help with R urgently?

I need to download the adespatial package, but the connection with the server is giving me problems and I am halfway stuck. I can download it manually but it has many add-ons that it is very...

02 March 2018 9,938 0 View

How to graph the components of the beta.div.com function of the adespatial package?

I need to make a triangular graph, I calculated the different parameters of the beta diverity using the beta.div.comp function of the adespatial package, but I do not know how to do the...

02 March 2018 1,593 1 View

How to make triangular graphics in R?

with the adespatial package, function beta.div.com, I found the components of beta diversity, but how do I graph that in a triangular graph ?. Any suggestions, Thanks

02 March 2018 1,119 0 View

Can someone identify this?

It was found on the surface of a Halimeda sp. in a shallow and rocky area. It was not strongly attached to the food but did not fall after washing with running water. Someone has seen something...

01 February 2018 9,077 2 View

What can a Photon Systems Instruments be used for?

In what types of experiment can I use the photon system instrument, if you have any article please send it to me or quote it

01 February 2018 4,746 1 View

Is there any report of fungi or endophytic bacteria causing diseases in algae or macroalgae meadows or marine ecology?

How can one identify the affectations in algae produced by parasites? How can we identify the effects on algae caused by fungi?

01 February 2018 9,576 2 View

Is ecological modeling possible at different spatial resolutions?

Sometimes when it comes to modeling it is necessary to compare layers from several sites and define which is the best. There are also cases in which we need layers from several sites because they...

31 December 2017 4,986 1 View

How much does a biologist know about signal processing, machine learning, and (theoretical) computer science?

I saw this questions and I need opinions about this!!! How much does a biologist know about signal processing, machine learning, and (theoretical) computer science?Anyway, given a typical PhD...

01 January 1970 3,900 3 View

Micro-Computed Tomography is a advance for underestend the life?

01 January 1970 8,470 2 View

Journals removed from JCR by high levels of self-citation and by concentration of citations between journals

This year, Clarivate Analytics, the editors of the Journal Citation Reports (JCR), deleted 20 journals, 14 for high levels of self-citation and six for the stacking of citations, a pattern...

01 January 1970 2,162 5 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View