Please look at the following below links which may help you in your analysis:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3503346/pdf/cn3000982.pdf

Thanks

Dear Demet,

about 12 years ago we established a strategy where a soluble pre-peptide was synthesized which which was well soluble in acidic buffer or DMSO at higher concentrations ( about 1 g/l) . This peptide could be added to a buffered cell culture medium in the desired dilution, where it changed to the biologically active form, which is usually only soluble in structure breaking solvents like trifluoroacetic acid (TFA) or hexafluoro isopronanole (HFIP) which cells do not like very much.

The colleagues joined the company Intavis peptides.

https://intavispeptides.com/

Maybe you can request a synthesis there, take aliquots of the freeze dried peptide, solubilize them in e.g. acetoc acid and add that solution to your final cell culkture medium in the desired dilution.

Best regards and good luck!

Dieter

Totally correct - many companies offer that peptide. Usually you can soubilize it in TFA or HFIP. But many biologist do not like to use such harsh solvents. The advantage of the peptide I descibed is that you get a soluble pre-peptide which forms the final amyloid peptide in the finale cell culture medium. So it is easier to handle.

Good luck

Dieter

Dear Demet Ünver thank you for asking this interesting technical question. Please have a look at the answers given to the following closely related question which has been asked earlier on RG:

I use Amyloid-β 25-35 and Amyloid-β 1-42 to induce neuroblastoma cells apoptosis. How to prepare these two peptide (now is lyophilized power )?

https://www.researchgate.net/post/I_use_Amyloid-b_25-35_and_Amyloid-b_1-42_to_induce_neuroblastoma_cells_apoptosis_How_to_prepare_these_two_peptide_now_is_lyophilized_power

(8 answers)

Also potentially useful is the following link:

Rapid aggregation and assembly in aqueous solution of Aβ (25–35) peptide

Article Rapid aggregation and assembly in aqueous solution of A β (2...

This paper has been posted as public full text on RG and can be freely downloaded as pdf file.

Good luck with your work! 👍.

Dear Dieter Stoll

First of all thanks for your answer. Many publications suggest that it is dissolved in deionized distilled water to prepare a stock solution and incubated at 37°C for 3 days. However, some studies have used TFA or HFIP. but as you said i don't want to use hard solvent for this study. I think I will use DMSO or distilled water to prepare the peptide. Thank you for your help,

Best regards..