Hello everbody. I just started my first scientif research and i'm having a little bit of trouble converting some units. I'm actually studing the possibily of building a biosensor to melatonine. I'm doing a review of what we already have in the literature about methods of detection, and I wanted to put every detection limit in pg/ml. For example, I found one that has a LD of " 1 fmol per well". I did some conversions (another question: I know the volume of a well is usely 350 uL, but there was this affirmation "the final volume of incubation was 126 uL", so I consider the volume of 126 uL. Am I right?), and found out that the limit were 6,67 pmol/L. Now I'm very confused of what I should do, because I don't have a cleary mass of melatonin (the objeticv of the research was to found that out in humam serum), just that they used 20 ng of melatonin-p-carboxybenzyl-AP conjugate, and I'm not so sure about what to do with this information. I hope this is not a very silly question. Thanks for the attention!
The mass of melatonin being 232 Da (wikipedia), a concentration of 1 pmol/l is equivalent to 0.232 pg/ml. If their assay volume is 126 µl, 1 fmol/well is equivalent to a concentration of 7.93 pmol/l or 1,84 pg/ml. The melatonin-AP (alkaline phosphatase ?) conjugate is used as a tracer in the assay to give a chromogenic reaction. I would advise you to consult your supervisor to explain the details of the assay, and possibly refresh your memory about molarity calculations.
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