I need to electroporate a 150 kb BAC into DH10B bacteria. Somebody do it? I can't achieve the right condition.
Are there open databases for mortality or large cardiovascular events such as Framingham? To be able to train predictive models of cardiovascular events with a large number of patients.
08 July 2024 6,133 2 View
Es posible observar que mientras que paises como colombia con altos indices de pobreza y desigualdad, abrieron sus fronteras a millones de migrantes venezolanos, por otro lado algunos paises de la...
16 June 2024 236 2 View
Existen diversos conflictos y problematicas sociales en el mundo que han causado migraciones. Por ejemplo, la migración venezolana y la reciente migración debido a la guerra entre ucrania y rusia....
16 June 2024 6,989 1 View
Has anyone seen MDR1 bands below 75 kDa? I am using a monoclonal antibody and I am expecting a band at 170 kDa
15 May 2024 8,690 4 View
I am preparing a drug for oral gavage which needs to be dissolved in 0.5% methylcellulose. The drug powder remains as clump and refuses to go into solution. Any tips on this?
01 April 2024 4,859 3 View
I'm a student researcher that is studying everything about the student's awareness on web browser and online behavior and safety practices. I wanted to know if there's a need of handout/mini book...
13 January 2024 8,315 5 View
If I want to transduce cells with two lentiviral vectors, do I perform the transduction one after the other? Or do I collect the lentivirus from the different HEK plates and co-transduce my target...
07 November 2023 7,632 5 View
Dear community, I’m trying to lyse Lysobacter enzymogenes cells. I want to open them up in a very gentle way to cause as little damage to everything that’s inside them (proteins, peptides,...
08 October 2023 2,045 0 View
Could be any scenario that you could find a higher EtCO2 than the value of a PCO2 of a ABG in a children with a congenic heart disease under invasive mechanical ventilation? In my case, a patient...
20 September 2023 7,746 1 View
Let's say I have SAV (~4spp) cover on a Braun-blanquet like scale for several years & I want to evaluate the effect of sediment characteristics, would a logistic model be the right approach? I...
16 July 2023 783 0 View
“Here is a thought experiment. Let's place Rodolpho Llinas's jarred-brain on top of a body (Fig. 1). I bet Llinas would argue that his jarred-brain retains its own consciousness, and the android...
11 August 2024 2,483 1 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...
08 August 2024 8,964 4 View
So I am organizing an AI seminar. What are possible AI projects in the AI for good spirit? something the students can do and have an impact?
08 August 2024 9,437 4 View
The rate of glucose consumption by the neocortex is reduced by over 80% during anesthesia (Sibson et al. 1998), which disables the synapses (Richards 2002) that are inundated by glial tissue (Engl...
08 August 2024 3,118 0 View
How to design human-centered classroom in the age of A.I.?
08 August 2024 347 5 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Do experts have journals in the field of artificial intelligence and big data that are not indexed by SCI or EI?
05 August 2024 8,836 2 View
Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...
05 August 2024 1,238 2 View
What's the role of IT & AI in Telecommunication Industry?
05 August 2024 8,264 3 View