What is the systemic procedure to study the process of a biochemical changes in a living plant? How these changes effect the physiology of plant? Can you also suggest some references?
This is a very 'broad' question. Which organ and which specific 'biochemical changes' are you looking for?
Yes Vinesh. Dont mind. Its a broad question as said by Yau sir.
What are you looking for has to be mentioned.
We generally I'll tell you what you can do is do tissue culture. Take tissues at each stage and check the biochemical changes. Biochemical changes can be in a wide range from syntheisis of biologically activr molecules to expression/supression of a gene.
For studying biochemical changes there are several methods that you can apply - generally we concentrate on bio-molecules and enzymes. For both you require to take tissue samples from plants, like leaf, stem, root, flower; extract the required bio molecule or enzyme quickly (or store tissue samples after freeze drying in liquid nitrogen in some cases)and quantify using well established assays.
I dont understand what you mean by 'living', because for these estimates you have to kill the tissue. If you are interested in non-invasive methods then there is also some like estimation of photosynthetic performance using MIN PAM or DUAL PAM etc.
You can also collect the plant material at different stages of development and qualify/quantify interesting metabolites in the samples, observig e.g. variable concentration depending on the maturity
or - as mentioned above - in different plant tissues
In such approach you just use the chosen analytical method
As suggested by Barbara, you can go with ontogenic changes of metabolites or enzymes. You can focus at the time of development of your interest. Further you may also think for in vivo estimation assay if any other internal compound may have influences on your interested chemical compounds.
The type or the group of plants u want to analyze is also important. Biochemical reactions may vary in different species of plants and are influenced by ecological and environmental conditions. However, there are many standard methods and procedures available for quantifying and measuring the synthesis of biomolecules.
Your question is too broad. Because the change of biochemical processes in plants could occur by many factors such as plant growth and development or changes in environmental conditions such as drought, microorganism infections and wind thus each of these have its own procedure for their study. Therefore, you must specify the purpose of the study and then, you should find the appropriate methods for that purpose. You can use in vivo assay for monitoring the changes of plant biochemical.
As an example of how this is possible, we use bio-luminescent reporters such as aequorin and luciferin to examine (and quantify in some cases) particular types of biological activity in whole, living plants. These reporters are genetically transformed into the species genome in previous generations, fused to promoters relevant to your requirements i.e. cell type specific or gene specific and thereby only expressed in a particular location and/or constitutively at a time of gene activation/promotion, allowing gene or protein activity to be assessed by luminescence.
- Badges
- Science topic
- Similar topics
- Biological Science
- Biota
- Eukaryota
- Viridiplantae
- Streptophyta
- Plants